Tissue Culture Laboratory

 

Production of Quality Sugarcane using Tissue Culture and Seed Treatment Mechanisms

Through meristem culture (tissue culture) and heat therapy, we produce disease free foundation seeds of sugarcane and grow secondary and commercial nurseries in the farmers’ fields for commercial planting and thus run the three tier Nursery programme effectively.
This programme helps us to keep diseases (like grassy shoot, ratoon stunting and yellow leaf) at bay and sustain yields.
KIAAR has established tissue culture laboratory and produces about 50,000 no.s of plantlets per annum. Protocol for the micropropagation of promising varieities like Co 86032, CoC 671, Co 94012, Co 91010, Co 2001-15, Co 07015, CoVSI 03102, Co 09004, VSI 08005 and Co 10001 has been established. Micropropagated plantlets are planted in the KIAAR farm and cane obtained is used to produce single – eye- bud setlings. Thus produced setlings are supplied to the farmers.Production of Tissue Culture plantlets and supply of single – eye – bud setlings during the last three years is tabulated below.

YearProduction of TC Plantlets (No.s)Supply of Single Eye Bud Setlingsto Farmers [Produced from Tissue Culture cane(No.s)]
2015-1649,5226,49,814
2016–1740,0008,09,596
2017-1850,00012,00,000

Details of media standardized for multiplication and rooting in micropropagation of sugarcane

Variety Multiplication media Rooting media
Co 86032 MS + BAP (2.0 ppm) MS + NAA (2.0 ppm)
CoC 671 MS + BAP (2.0 ppm) MS + IBA (4.0 ppm)
Co 94012 MS + BAP (1.0 ppm) MS + NAA (2.0 ppm)
Co 91010 MS + BAP (1.0 ppm) MS + IBA (1.0 ppm) + GA3 (1.0 ppm)
Co 2001 – 15 MS + BAP (0.2 ppm) + KN (0.1 ppm) MS + NAA (2.0 ppm)
Co 07015 MS + BAP (2.0 ppm) MS + NAA (2.0 ppm)
CoVSI 03102 MS + BAP (2.0 ppm) MS + IBA (2.0 ppm)
Co 09004 MS + BAP (2.0 ppm) MS + IBA (2.0 ppm)
VSI 08005 MS + BAP (2.0 ppm) MS + IBA (2.0 ppm)
Co 10001 MS + BAP (2.0 ppm) MS + IBA (2.0 ppm)