Production of Quality Sugarcane using Tissue Culture and Seed Treatment Mechanisms
Through meristem culture (tissue culture) and heat therapy, we produce disease free foundation seeds of sugarcane and grow secondary and commercial nurseries in the farmers’ fields for commercial planting and thus run the three tier Nursery programme effectively.
This programme helps us to keep diseases (like grassy shoot, ratoon stunting and yellow leaf) at bay and sustain yields.
KIAAR has established tissue culture laboratory and produces about 50,000 no.s of plantlets per annum. Protocol for the micropropagation of promising varieities like Co 86032, CoC 671, Co 94012, Co 91010, Co 2001-15, Co 07015, CoVSI 03102, Co 09004, VSI 08005 and Co 10001 has been established. Micropropagated plantlets are planted in the KIAAR farm and cane obtained is used to produce single – eye- bud setlings. Thus produced setlings are supplied to the farmers.Production of Tissue Culture plantlets and supply of single – eye – bud setlings during the last three years is tabulated below.
|Year||Production of TC Plantlets (No.s)||Supply of Single Eye Bud Setlingsto Farmers [Produced from Tissue Culture cane(No.s)]|
Details of media standardized for multiplication and rooting in micropropagation of sugarcane
|Variety||Multiplication media||Rooting media|
|Co 86032||MS + BAP (2.0 ppm)||MS + NAA (2.0 ppm)|
|CoC 671||MS + BAP (2.0 ppm)||MS + IBA (4.0 ppm)|
|Co 94012||MS + BAP (1.0 ppm)||MS + NAA (2.0 ppm)|
|Co 91010||MS + BAP (1.0 ppm)||MS + IBA (1.0 ppm) + GA3 (1.0 ppm)|
|Co 2001 – 15||MS + BAP (0.2 ppm) + KN (0.1 ppm)||MS + NAA (2.0 ppm)|
|Co 07015||MS + BAP (2.0 ppm)||MS + NAA (2.0 ppm)|
|CoVSI 03102||MS + BAP (2.0 ppm)||MS + IBA (2.0 ppm)|
|Co 09004||MS + BAP (2.0 ppm)||MS + IBA (2.0 ppm)|
|VSI 08005||MS + BAP (2.0 ppm)||MS + IBA (2.0 ppm)|
|Co 10001||MS + BAP (2.0 ppm)||MS + IBA (2.0 ppm)|